Intravital Cinemicrostrob: A Vertical Optical Bench for Epicondenser Reflected Dark Field Illumination

A prototype optical bench with axial alignments (for epicondenser reflected light microscopy at a depth within intact living tissues) with servo electronic mechanisms, adequate for modeling and for freeze flash color cinemicroscopy of the microcirculation of the lung has been constructed and operates at up to 400 frames/s. The pulmonary arteriolar and capillary blood flow is observed in open chest, still-lung rabbit preparation. Experimental “meconium embolism” serves as a control for “fibrination.” These simulate, respectively, microcirculatory disorders that occur spontaneously during two opposite, acutely acquired accidents of late human pregnancy: microembolism of debris during amniotic embolism and disseminated coagulation during abruptio placentae.

Print ISSN

0361-4573

Published

1967-08

Content type

Original Research

DOI

10.5594/J09126