Holographic Motion-Picture Microscopy: A Reprint

Cameron Knox, Robert E. Brooks

Holographic motion pictures provide a means for recording and observing transient, dynamic, small-scale events occurring at unpredictable times and locations in a given volume of space. Using an argon ion laser to generate repetitive pulses of 50μs duration at a wavelength of 514.5 nm (5145 Å), holographic movies of living marine plankton organisms were produced by recording a series of in-line holograms at 70 frames/s on 35mm film. The reconstructed holographic images may be viewed with a standard microscope, photographed with a still camera, or copied with a conventional movie camera for subsequent projection. Image resolution of 10 μs was obtained.

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Published
1970-07
Content type
Original Research
DOI
10.5594/J14321